BIOSENSORS FOR RAPID DETECTION OF INSECT RESISTANCE AND PARENTAL IDENTIFICATION IN CROPPLANTS.

7 students

Objectives:

  1. To detect Pink bollworm resistance in Bt. cotton using immunologicalassay.
  2. To develop enzyme based molecular kit for identification of Bollwormcomplex.
  3. To test hybrid purity and identify spurious seeds in crop plants by using DNA based biosensors.
Brief contents:

The course will help in fast, reliable and effective identification four significant pest species in the cotton bollworm complex (Helicoverpa armigera, Helicoverpa assulta, Pectinophora gossypiella and Earias vittella) irrespective of their life stages and will offer support to conventional taxonomic differentiation based on morphological characters. Similarly, in recent years Pectinophora gossypiella (Pink bollworm) become resistant to first generation transgenic Bt cotton, which threatened the cotton growers and brain workers in area of biotechnology. This project will help in developing of immunological assay to analyze Btgeneexpression.

Secondly, students will get hands on training on DNA based identification of spurious seeds in crop plant. This will help in developing entrepreneurs in field of biotechnology for detecting insect resistance and pure seed identification at initiallevel

Part-1

Assessment and analysis of cotton bollworms from Bt cotton and non Bt. Cotton fields for developing DNA based diagnostic tool in early identification. The primary work of standardizing mitochondrial DNA isolation protocol followed by amplification of two partial regions of the mitochondrial DNA (mtDNA), the cytochrome oxidase subunit I (COI) and the cytochrome b (Cyt b) genes by PCR and digested with restriction endonucleases. This will leads in generation of DNA patterns for differentiation of the four pest species.

Part-2

Development of Immuno-biosensors for analysis to study intra-plant and in-seasonal variability in Cry1Ac expression in Btcotton. The gene expression in plant parts will be correlated with survival of pink bollworm on different plant parts so as to quantify the variation of gene expression and infestation of pink bollworm.

Part-3

The different molecular markers will be used to develop PCR based biosensors in crop plants. The PCR based biosensors will help in parental identification, detection of spurious seeds and verification of hybrid purity in crop plants. This technique is fast, reliable and effective at distinguishing specimens irrespective of their life stages.

Expected outcome:

By the end of the course students / participants will acquire the knowledge of;

  1. Development of molecular diagnostic kit for identification of cotton bollworms at earlystage.
  2. Help in addressing farmers complaints related to bollworm survival on Bt-cotton plants as study makes a systematic attempt to correlate the Cry1Ac expression in Bt cotton tissues with pink bollworm mortality, thus identifying a critical expression level for Cry1Ac in tissues, below which insect would be able tosurvive.
  3. Development of new entrepreneurs in area of biotechnology which may become a part of agricultural clinic as PCR biosensors will help in fast, reliable and effective detection of insect pest irrespective of their life stages and seed purity.
Curriculum is empty

Instructor

Master in Agriculture sciences having India and abroad working experince in Horticulture industry.

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